Molecular Pathology of Liver Diseases by Allan Tsung, David A. Geller (auth.), Satdarshan P. S. Monga

By Allan Tsung, David A. Geller (auth.), Satdarshan P. S. Monga (eds.)

Molecular Pathology of Liver ailments integrates the conventional wisdom of physiological and pathological tactics within the liver with a balanced emphasis on basic innovations; well timed advances in mobile and molecular mechanisms; and utilized pathology. The textbook is prepared into numerous sections, every one of including an array of chapters that steadily and cohesively complicated on pertinent liver biology and pathology. the 1st 3 sections speak about the mobile composition of the liver in addition to their really good services, and extra dissect the molecular foundation of the mobile approaches which are so distinct to the liver. the following part examines the mechanisms which are mostly implicated within the mobile and molecular foundation of a number of hepatic pathologies, ultimately through a piece each one on a mess of non-neoplastic and neoplastic ailments of the liver. Molecular Pathology of Liver ailments is a accomplished reference on liver pathobiology for simple, translational and scientific researchers and physicians The layout of the quantity will function a prepared connection with correct subject matters within the liver, hence supplying a realistic disease-based integrative source at the molecular pathology of liver disorder. Satdarshan (Paul) Singh Monga, MD is Director: department of Experimental Pathology and affiliate Professor of Pathology and drugs, collage of Pittsburgh, Pittsburgh, PA

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For storage, isolated hepatocytes can be resuspended in ViaSpan or cryopreserved using dimethyl sulfoxide (DMSO). Viability is usually assessed based on the ability of cells to exclude trypan blue. Unfortunately, this measure is a poor guide to hepatocyte function and engraftment potential. In vitro characteristics of isolated hepatocytes have not been shown to correlate with engraftment, and the ability of the cells to adhere to tissue culture plates 24 h after seeding is a better in vitro assay of engraftment potential [12].

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1995;21(6):1658–67. Gebhardt R, Baldysiak-Figiel A, Krugel V, Ueberham E, Gaunitz F. Hepatocellular expression of glutamine synthetase: an indicator of morphogen actions as master regulators of zonation in adult liver. Prog Histochem Cytochem. 2007;41(4):201–66. Gebhardt R, Lindros K, Lamers WH, Moorman AF. Hepatocellular heterogeneity in ammonia metabolism: demonstration of limited colocalization of carbamoylphosphate synthetase and glutamine synthetase. Eur J Cell Biol. 1991;56(2):464–7. Haussinger D, Lamers WH, Moorman AF.

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