Gigaseal Formation in Patch Clamping: With Applications of by Majid Malboubi, Kyle Jiang (auth.)

By Majid Malboubi, Kyle Jiang (auth.)

This ebook provides an research of gigaseal formation utilizing micro/nanotechnology. The goals of the publication are twofold. First, it explains the mechanisms of gigaseal formation utilizing the newest discoveries. moment, it presents useful ideas for widespread formation of excessive resistance seals. The formation of a high-resistance electric seal, often referred to as a gigaseal, among a cellphone membrane and a tumbler micropipette tip is vital in patch-clamp experiments. even supposing 4 a long time have handed because the creation of the patch-clamping process by way of Neher and Sakmann, gigaseal formation continues to be a drawback in constructing the high-throughput ion channel screening structures required via the pharmaceutical undefined. the following the authors percentage their newest tools for reaching gigaseal formation and describe options which are hugely fascinating at either learn and business degrees. Nanotechnology has been stumbled on to be a robust software for learning and enhancing glass micropipettes and in tackling the matter of gigaseal formation.

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4. The seal is distributed over the pure lipid bilayer region. As was discussed earlier, the resistance pure unit length of area of membrane-glass contact is very high. 5. Some studies have claimed that the close opposition of lipid bilayer and glass cannot be obtained in the presence of integral proteins. In this model membrane proteins protrude above the bilayer by a few nanometres. 8). Therefore this model shows that a gigaseal can be obtained while the proteins are present in the seal. Because of the denaturation process of membrane proteins the seal happens gradually.

Petrov AG (2001) Flexoelectricity of model and living membranes. Petrov AG (2006) Electricity and mechanics of biomembrane systems: flexoelectricity in living membranes. Lau AY et al (2006) Open-access microfluidic patch-clamp array with raised lateral cell trapping sites. Lab Chip 6:1510–1515 Chapter 4 Effect of Roughness on Gigaseal Formation Surface roughness is one of the most important factors in gigaseal formation and its effect has been emphasized in the literature [1–8]. A rough pipette tip in conventional patch clamping, or patching site in planar patch clamping prevents seal formation.

3, ρm = 1150 kg/m3 smoother tips resulted in higher seal values. Thus, FIB-polished glass micropipettes have improved the gigaseal formation in patch clamping. Malboubi M, Gu Y, Jiang K (2010) Study of the tip surface morphology of glass micropipettes and its effects on giga-seal formation. Electronic engineering and computing technology. Malboubi M et al (2009) Effects of the surface morphology of pipette tip on giga-seal formation. Malboubi M et al (2009) The effect of pipette tip roughness on giga-seal formation.

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