Advances in Enzymology and Related Areas of Molecular by Alton Meister

By Alton Meister

A continuous authoritative sequence reviewing learn into enzymology and similar parts of molecular biology. offers six papers by way of top professionals.


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A chalcone cyclase (isomerase) with very different properties has been isolated from Citrus paradisi. In order to act as substrate, the chalcone requires an unhindered B-ring hydroxyl group [unlike the enzyme from P. vulgaris (183)] and the 4’ position must be glycosylated via @-linkedneohesperidose (1 85). Chemical models for the synthesis of isoflavonoids have predicted direct formation of isoflavones from chalcones (1 86,187), and this would imply n o direct role for CHI in the synthesis of isoflavonoid phytoalexins.

Such a possibility awaits further investigation. In spite of the important position of CHS as the first committed step in flavonoid/isoflavonoid biosynthesis, few studies have been made on its activity in plant cells producing phytoalexins. In cotyledons of Glycine mar, CHS activity was induced by treatment with a cell wall suspension prepared from mycelium of Phytophthom megaspema fspglycinea (151). The enzyme activity reached a maximum level by about 25 hr after treatment; this increase was subsequent to the induction of PAL, but it slightly preceded the accumulation of glyceollin (XIV).

Infestans G. cingulata. -WOH Ho ‘ . 277) (252,256,274, 275) (27 2,27 3) (254,27 1) (254) 1)1 P (279,289) Fusarium (LXXX) Capsinone oxyspomrn (281,287,288) M. fmcticola (LXXVII) Capsidiol (282-286) Sweet pepper (Capsicum fmtescens) Tobacco necrosis virus and bacteria (278,279) (LXV) (LXXVII) (LXXIX) M. 3-Dihydroxygermacrene (267,278,279) M. fmcticola (LXVIII) (261,274,218) Hydroxylubimin Capsidiol References Fungal species M. ) Jimsonweed (Datura stramonicum ) Source TABLE V (CONTINUED) cossypol (R,R' = OH) (LXXXVII) (LXXXVI) Momilactone B Cotton ( Gossypium SPPJ (LXXXV) Momilactone A (LXXXIII) Dehydroipomeamarone Rice (Oryza sativa) (LXXXII) lpomeamaronol Casbene (LXXXI) lpomeamarone Castor (Ricinus communis) Sweet potato (Ipomea batatas) (294,295) C.

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