By Richard Kordal, Arthur Usmani, Wai Tak Law
content material: Gene expression microarrays: a platform for discovery in organic platforms / Karen Seta ... [et al.] --
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AlphaScreen: a hugely delicate, nonradioactive and homogeneous assay platform for drug discovery excessive throughput screening, genomics, and lifestyles technology learn purposes in miniaturized layout / Changjin Wang ... [et al.] --
Light-powered microtransponders for prime multiplex-level analyses of nucleic acids / Wlodek Mandecki ... [et al.] --
DNA biosensor: immunosensor functions for anti-DNA antibody / Koji Nakano ... [et al.] --
electronic optical chemistry: a singular approach for the fast fabrication of customized oligonucleotide arrays / Kevin J. Luebke ... [et al.] --
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Acknowledgements I would like to thank all of my colleagues, who are authors of the papers cited, for stimulating discussions. S. Department of Energy, under Contract No. W-31-109-ENG-38 and by grant 5/2000 from the Russian Human Genome Program. References 1. Reviews: The Chipping Forecast. Nature Genetics 1999, 21, 1-60. 2. , and Mirzabekov, A . Advance in the analysis of D N A sequence variations using oligonucleotide microchip technology. Current Opin. , 2001, 12(1), 53-58. 3. Lysov, Y u . ; Florentiev, V .
Patel, and H . Kirakossian of Dade Behring, Inc. (San Jose, California), for many valuable advises and discussions. References 1. 2. 3. 4. ; Farr-Jones, S; Yund, M. J. Biomol. Screen. ; Hertzberg, R. J. Biomol. Screen. 1999, 4, 231-234 Fernandes, P. Curr. Opin. Chem. Biol. ; Burbaum, J. J Biomol. Screen. 2000, 5, 177-187 5. ; Oldenberg, K . J. Biomol. Screen. 1999, 4, 137141 6. ; Terstappen, G . J. Biomol. Screen. 1999, 4, 3-7 7. ; Menard, L. J. Biomol. Screen, 1998, 3, 285-292 8. ; Hermes, J. Mol.
Non-proximity based homogeneous assays, such as fluorescence polarization (FP) (11) and fluorometric microvolume assay technology (FMAT) (12), have also emerged into HTS applications. Not all assay technologies, however, are suitable for miniaturization, primarily due to signal intensity. ; ACS Symposium Series; American Chemical Society: Washington, DC, 2002. 47 thus can be miniaturized, is cost effective, and can be used for broad applications in drug discovery HTS, general life science research and even genomics.